TABLE 4.
Primers related to segment-specific PCR
| Reaction | Target | Primer name | Sequence (5′→3′) |
|---|---|---|---|
| RT | All segments (WT) | Uni12 | AGCAAAAGCAGG |
| Segment 7 (OP7 virus) | S7-OP7 RT | AAGCAGGTAGATATTGAAAG | |
| PCR | Segment 1 | S1 Uni for | AGCGAAAGCAGGTCAATTAT |
| S1 Uni rev | AGTAGAAACAAGGTCGTTTTTAAAC | ||
| Segment 2 | S2 Uni for | AGCGAAAGCAGGCAAACCAT | |
| S2 Uni rev | AGTAGGAACAAGGCATTTTTTCATG | ||
| Segment 3 | S3 Uni for | AGCGAAAGCAGGTACTGATCC | |
| S3 Uni rev | AGTAGAAACAAGGTACTTTTTTGG | ||
| Segment 4 | S4 Uni for | AGCAAAAGCAGGGGAA | |
| S4 Uni rev | AGTAGAAACAAGGGTGTTTT | ||
| Segment 5 | S5 Uni for | AGCAAAAGCAGGGTAGATAATC | |
| S5 Uni rev | AGTAGAAACAAGGGTATTTTTC | ||
| Segment 6 | S6 Uni for | AGCGAAAGCAGGGGTTTAAAATG | |
| S6 Uni rev | AGTAGAAACAAGGAGTTTTTTGAAC | ||
| Segment 7 | S7 Uni for | AGCGAAAGCAGGTAGATATTG | |
| S7 Uni rev | AGTAGAAACAAGGTAGTTTTTTAC | ||
| Segment 7 (OP7 virus) | S7-OP7 PCR for | AAGCAGGTAGATATTGAAAG | |
| S7-OP7 PCR rev | AGTAGAAACAAGGTAGTTTT | ||
| Segment 8 | S8 Uni for | AGAAAAAGCAGGGTGACAAA | |
| S8 Uni rev | AGTAGAAACAAGGGTGTTTT | ||