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. 2019 Feb 5;93(4):e01457-18. doi: 10.1128/JVI.01457-18

FIG 7.

FIG 7

Cytosolic to nuclear relocalization of HBcAg does not alter HBc18-27 epitope presentation. (a) Cytoplasmic distribution of HBsAg at days 7 and 28 postinfection in HepG2-hNTCP-A3 cells using confocal laser scanning microscopy. HBsAg was stained in green using anti-HBs antibody while the nucleus was stained blue using DAPI. (b) HBcAg relocalization from cytosolic (days 7 and 14 postinfection) to more nuclear localization (21 and 28 days postinfection) during HBV infection in HepG2-hNTCP-A3 cells using confocal laser scanning microscopy. HBV-infected HepG2-hNTCP-A3 cells were stained with anti-HBc antibody (red), and DAPI (blue) was used to stain the nucleus. (c) Ability of CD8+ T cells specific for HBc18-27 to recognize HepG2-hNTCP-A3 cells infected with HBV for the indicated time. Shown is the frequency of the CD8+ T cells positive for CD107a among total CD8+ T cells upon coculture with HepG2-hNTCP-A3 targets for 5 h at an E/T ratio of 1:2. Data are shown as means ± standard deviations of at least two independent experiments. (d) Direct quantification of A2-HBc18 complexes on the surface of HBV-infected HepG2-hNTCP-A3 cells kept in culture for a prolonged duration of infection. Bars represent the MFI values of A2-HBc18 on HBV-infected HepG2-hNTCP-A3 cells normalized to the value in uninfected cells at each time postinfection. MFI values were measured using an ImageStream analyzer.