FIG 1.

Depletion of CD4⁺ T cells from peripheral tissue and secondary lymphoid tissue of HSV-2-infected guinea pigs by treatment with anti-guinea pig CD4 MAb. (A) Illustration of CD4 depletion regimen. Female guinea pigs received anti-CD4 MAb or diluted rat serum as a control treatment beginning on day 21 after HSV-2 inoculation and on days indicated by red arrows. Lymphocytes from representative animals of each treatment group were harvested on day 25 p.i. or at the termination of the experiment, between days 39 and 45 p.i. (B) Representative histograms demonstrating the gating strategy for analysis of CD4⁺ and CD8⁺ T lymphocyte populations. (C) Representative histograms of T lymphocyte populations from a CD4-depleted and control-treated animal. Results are from a single experiment, representative of three identical experiments performed.