FIG 3.

HSV-specific ASC responses and serum antibody levels are not diminished by CD4 depletion. Sera and lymphocytes from spleen, bone marrow, and ingLN were obtained from guinea pigs after the termination of the CD4 depletion experiment (between days 39 and 45 p.i.). (A to C) The number of HSV-specific ASC in the indicated tissues of individual animals was quantified by ELISPOT assay, and the results are reported as the mean number of HSV-specific ASC/tissue ± standard errors of the means (SEM). Results represent data pooled from two identical experiments, n = 9 to 17. (D) The titer of serum HSV-gD-specific antibody was determined in individual animals by ELISA and reported as the mean endpoint dilution titer ± SEM (log₁₀). Results represent data pooled from three identical experiments (n = 7 naive, 27 CD4-depleted, and 22 control-treated animals). *, P < 0.0001 (ANOVA with Tukey’s multiple-comparison test).