FIG 4.

Frequency of HSV-specific, CD8⁺ IFN-γ SC is significantly diminished by depletion of CD4⁺ T cells. (A) Total number of IFN-γ SC in spleens of CD4-depleted and control-treated animals obtained after the termination of the depletion experiment (between days 39 and 45 p.i.). Results from individual animals were determined and expressed as the mean number of IFN-γ SC per spleen ± SEM. Results represent data pooled from two identical experiments (n = 14 CD4-depleted and 12 control-treated animals). (B) Frequency of CD8⁺ HSV-specific, IFN-γ SC in spleens of CD4-depleted and control-treated animals. Splenic CD8⁺ lymphocytes were enriched by antibody-coated beads and the number of HSV-specific IFN-γ SC determined by ELISPOT assay. Results from individual animals (n = 8 CD4-depleted and 8 control-treated animals) were determined and expressed as the mean number of CD8⁺ IFN-γ SC per 10⁶ enriched CD8⁺ T lymphocytes ± SEM.