FIG 6.

Inhibition of PRRSV SD16 infection and replication by TAT-NB6 in PAMs. PAMs were infected with SD16 at an MOI of 0.01 for 1 h, and then the cell culture media were replaced with fresh RPMI 1640 containing 3% FBS and TAT-Nbs at the indicated concentrations. TAT-Nbs and PRRSV Nsp9 were detected at 24 hpi (A) and 36 hpi (C) by Western blotting using anti-His MAb and mouse anti-Nsp9 antiserum, respectively. Progeny virus released in the cell medium was measured by TCID₅₀ at 24 hpi (B) and 36 hpi (D). (E) Relative levels of PRRSV RNA were detected by RT-qPCR using PRRSV Nsp9-specific primers. The GAPDH mRNA level served as an internal reference. Data are expressed as means ± SD from three independent experiments. P values were calculated using ANOVA as <0.05 (*), <0.01 (**), and <0.001 (***) compared with cells infected with PRRSV alone (ns, not significant).