FIG 7.

TAT-NB6 inhibits the replication of multiple PRRSV strains. PAMs were infected with JXA1, SD16, GD-HD, VR2332, or GZ11-G1 at an MOI of 0.01 and incubated with 30 μM TAT-NB6 or TAT-NB53. The transcription levels of PRRSV Nsp9 in the cells were detected via RT-qPCR at 12 (A), 24 (B), 36 (C), and 48 (D) hpi. (E) The titers of progeny virus in the cell culture supernatants were measured by TCID₅₀ at 48 hpi. Data are expressed as means ± SD from three independent experiments. P values were calculated using ANOVA as <0.05 (*), <0.01 (**), and <0.001 (***) compared with cells infected with PRRSV alone (ns, not significant).