TABLE 1.
Primers used for construction of plasmid pET-21b-TAT-Nbs or pET-21b-Nbs and detecting the level of PRRSV Nsp9 gene by qPCR
| Primer name | Sequencea (5′–3′) | Purpose |
|---|---|---|
| Overlap-TAT-Nb-F1 | CCGCATATGCCTACGGTCGTAAGAAACGTCGCC | pET-21b-TAT-Nbs |
| AGCGTCGCCGTGGAGGCGGTGGCTCGGG | ||
| Overlap-TAT-Nb-F2 | CGGTGGCTCGGGCGGTGGCGGCTCGGGTGGCGG | |
| TGGTTCTCAGGTCCAACTGCAGGAG | pET-21b-Nbs | |
| Nb-F | CCGCATATGCAGGTCCAACTGCA | |
| Nb-R | CCGCTCGAG TGAGGAGACGGTG | |
| Type1-Nsp9-F | GTCTTGAGGCTGACTTGGCT | RT-qPCR |
| Type1-Nsp9-R | TTGGACACACTGGTAACGGG | |
| Type2-Nsp9-F | CCTGCAATTGTCCGCTGGTTTG | |
| Type2-Nsp9-R | GACGACAGGCCACCTCTCTTAG | |
| GAPDH-F | CCTTCCGTGTCCCTACTGCCAAC | |
| GAPDH-R | GACGCCTGCTTCACCACCTTCT |
a
Underlining indicates restriction sites.