FIG 2.
Visualization of Fab-decorated HPeV3. Virus-Fab reconstruction shown radially color coded (purple, 100 Å; yellow, 120 Å; light blue, 140 Å; blue, 145 Å; and orange, 150 Å). (A) Surface view down a 2-fold axis of symmetry. Propeller regions can be seen as a dark blue triangles on the capsid surface, the Fabs are orange. (B) Cutaway view showing RNA (purple) at 5-fold vertices inside the viral capsid. (C) Central cross-section with well-defined layers of density corresponding to RNA, capsid, and Fab. (D) Side chains for viral coat proteins VP0 (blue), VP1 (green), and VP3 (light blue), as well as Fab heavy and light chains, VH (orange) and VL (light brown), respectively. (E) Modeled asymmetric unit with a Fab molecule bound. (F) High resolution at the Fab AT12-015-HPeV3 interface. (G) RNA anchoring on the inner surface of the virus is mediated by a tryptophan (Trp 24) residue from VP3.