TABLE 1.
Summary of cryo-EM data collection, refinement, and validation statistics
| HPeV3-Fab complex data categorya | Value |
|---|---|
| Data collection | |
| Voltage (kV) | 300 |
| Electron exposure (e−/Å2 · s) | 48 |
| Pixel size (Å) | 1.06 |
| No. of micrographs | 6,541 |
| Reconstruction | |
| No. of particles | 74,927 |
| B factor (Å2) | −70 |
| FSC threshold | 0.143 |
| Resolution (Å) | 2.8 |
| Model building | |
| VP0 (amino acid coverage) | 34–289 |
| VP1 (amino acid coverage) | 25–219 |
| VP3 (amino acid coverage) | 16–256 |
| vRNA (nt) | 8 |
| VH | 2–119 |
| VL | 2–109 |
| Model validation | |
| MolProbity score | 1.55/96th percentile (protein) |
| Ramachandran outliers (%) | 0.93 (protein) |
| Poor rotamers (%) | 3.91 (protein) |
| Clashscore | 0 (protein), 4.35 (vRNA) |
a
FSC, Fourier shell correlation; nt, nucleotide; VH, Fab heavy chain; VL, Fab light chain.