FIG 10.

G₂/M arrest inhibits upregulation of ISG expression in response to exogenously added IFN-α. (A) Suit2 cells were mock treated or treated for 24 h with 500 nM paclitaxel or 500 nM colchicine and then stimulated with 5,000 U/ml IFN-α for 4 h. Three biological repeats were conducted on 3 different days under each condition for RNA microarray analysis. Three comparisons were done (control plus IFN, paclitaxel plus IFN, and colchicine plus IFN versus control, paclitaxel, and colchicine, respectively). The number of transcripts upregulated in response to IFN-α treatment is indicated for each comparison in the Venn diagram. (B) List of the commonly upregulated transcripts in all three comparisons. (C) Suit2 cells were treated (or mock treated) with 500 nM paclitaxel for 24 h with or without 5,000 U/ml IFN-α and then infected with serial dilutions of VSV-ΔM51 and incubated for 24 h to calculate virus infectivity under each condition. The data represent the means and SD of the means from three independent experiments. ***, P < 0.001; ****, P < 0.0001. The significance of the data was determined by using one-way ANOVA with a Tukey posttest at a 95% confidence interval for comparisons of VSV-ΔM51 versus VSV-ΔM51 plus IFN, VSV-ΔM51 plus paclitaxel versus VSV-ΔM51 plus IFN, and VSV-ΔM51 plus paclitaxel plus IFN versus VSV-ΔM51 plus IFN.