FIG 8.

ASS1 does not regulate the expression of GLUT1 and GLUT3, and glucose deprivation does not affect ASS1 protein and intracellular NO levels. (A) ASS1 knockdown has no effect on the expression of GLUT1 and GLUT3 proteins. MM and KMM cells transduced with 3 ASS1 shRNAs (shRNA1, -2, or -3) or a scrambled shRNA (ctl) for 2 days were examined for the levels of GLUT1 and GLUT3 proteins. (B) Overexpression of ASS1 has no effect on the expression of GLUT1 and GLUT3 proteins. MM and KMM cells overexpressing ASS1 or a vector control (V) for 2 days were examined for the levels of GLUT1 and GLUT3 proteins. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (C and D) Glucose deprivation does not affect ASS1 protein and intracellular NO levels. MM and KMM cells were seeded overnight in full medium, cultured in medium with and without glucose for 12 and 24 h, and examined for the level of ASS1 protein (C) or intracellular NO (D).