FIG 6.

Trivalent vaccine sera bind linear EBOV GP peptides similarly to single-component vaccine sera. Sera from animals vaccinated with either HPIV3-EBOV (A), HPIV3-BDBV (B), HPIV3-SUDV (C), or the trivalent vaccine (D) were analyzed for binding to peptides matching the protein sequence of EBOV GP. Binding of sera collected on day 28 (gray) is overlaid on binding of sera collected on day 56 (color) to highlight regions that showed increased binding after the second vaccine dose. Values represent the average binding of sera from 3 vaccinated animals as a percentage of the binding of sera drawn prior to vaccination. Each column represents the results for a 15-residue peptide with a sequence matching the sequence of EBOV GP. The diagrams below the graphs show the domains of GP in a linear fashion that correspond to the binding data. S, signal peptide; blue, base; yellow, head; orange, glycan cap; dotted region, mucin domain; green, fusion loop; light gray, HR1; dark gray, HR2; red, transmembrane and cytoplasmic tail. The furin cleavage site is marked by the triangle.