Figure 2. The weight of nude mice (mean ± SE) was evaluated before and after treatment with MET alone, VPA alone, MET plus VPA, or vehicle control (n=3). There was no significant difference in weight between the groups over 8 weeks of treatment (p>0.05) (A). Representative hematoxylin and eosin staining images (10Χ and 40Χ) of the kidney (B) and the liver (C), during week 8 of treatment. The histological scoring of the kidney (B) showed no tubule (t) changes, thrombosis, dilation, necrosis, or inflammation (score 0). The histological scoring of the liver (C) showed no signs of portal fibrosis around central vein (c), lobular inflammation or necrosis, Mallory bodies, hepatocyte ballooning, perisinusoidal fibrosis, or fatty changes (score 0). Scale bar 4 mm for 10Χ images, and 80 μm for 40Χ images.