Table 1.

Kinetic parameters for the interaction of L-/D-kalata mixtures with POPC/POPE membrane.

L-/D-kB1[a]	Initial rate [ngmm−2S−1][b]	Peptide/lipid [mol/mol][c]	kd [× 10−2S−1][d]
100:0	0.419	0.23	1.66 ± 0.01
75:25	0.330	0.20	1.62 ± 0.01
50:50	0.160	0.18	1.79 ± 0.01
25:75	0.120	0.18	4.42 ± 0.03
0:100	0.068	0.08	1.84 ± 0.01

^[a]50 μM of total peptide was injected.

^[b]The initial peptide binding rate was calculated from the initial slope of the sensorgram (10 s after injection start); response units (RU) were converted into ngmm⁻² (1 RU ~1 pgmm⁻²).

^[c]Peptide/lipid ratio at the end of association phase. The amount of lipid was calculated by converting the RU obtained at the end of lipid deposition into ngmm⁻², while the amount of peptide bound was calculated at the end of injection (see Figure S3 for further details on the calculations).

^[d]The dissociation constant (k_d) was calculated by separately fitting the dissociation phase with the BIAeval software (version 4.1), the standard error is given.