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Effect of miR-494-3p on the synthesis and release of cytokines. Rat cardiomyocytes were transfected with miR-NC or miR-494-3p mimics following incubation with septic shock serum. (A) TNF-α and IL-6 protein expression was measured using western blotting. Concentrations of (B) TNF-α and (C) IL-6 in the culture supernatant were determined using ELISA. *P<0.05 vs. miR-NC group. miR, microRNA; TNF-α, tumor necrosis factor-α; IL-6, interleukin-6; NC, negative control.
