Table I.
Sequences of primers used for polymerase chain reaction.
| Gene | Sequence no. | Primer sequence (5′-3′) | Product length (bp) |
|---|---|---|---|
| PEPCK | NM_011044.2 | F, GTGCTGGAGTGGATGTTCGG | 258 |
| R, CTGGCTGATTCTCTGTTTCAGG | |||
| G6pase | NM_008061.4 | F, ACTGTGGGCATCAATCTCCTC | 344 |
| R, CGGGACAGACAGACGTTCAGC | |||
| FoxO1 | NM_019739.3 | F, AAGAGCGTGCCCTACTTCAA | 157 |
| R, CTCCCTCTGGATTGAGCATC | |||
| PGC-1α | NM_008904.2 | F, ATACCGCAAAGAGCACGAGAAG | 253 |
| R, CTCAAGAGCAGCGAAAGCGTCACAG | |||
| HNF4α | NM_008261.3 | F, ATGCGACTCTCTAAAACCCTTG | 135 |
| R, ACCTTCAGATGGGGACGTGT | |||
| β-actin | NM_007393.5 | F, GGCTGTATTCCCCTCCATCG | 154 |
| R, CCAGTTGGTAACAATGCCATGT |
FOX, forkhead box; PEPCK, phosphoenolpyruvate carboxykinase; G6pase, glucose 6-phosphatase; PGC1α, peroxisome proliferator-activated receptor γ coactivator 1α; HNF4α, hepatocyte nuclear factor 4α; F, forward; R, reverse.