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. 2019 Jan 9;17(3):2019–2030. doi: 10.3892/etm.2019.7163

Table I.

Primer sequences used for the genotyping analysis of RAGE and GAPDH variations.

Name Primer sequence (5′-3′) Size of PCR product (bp) Thermocycling protocol
RAGE F: AGGTGAGTGGAGAAAGCCAG 121 94°C for 3 min;
R: ATGTGTCAGGTGTTTAATCA 35 cycles of 94°C for 30 sec, 56°C for
30 sec and 72°C for 30 sec;
72°C for 7 min;
4°C ∞
94°C for 3 min;
GAPDH F: AGGATGGTGTGGCTCCCTTG 105 35 cycles of 94°C for 30 sec, 58°C for
R: GCAGGGCTGAGACAGCTTCC 25 sec and 72°C for 25 sec;
72°C for 7 min;
4°C ∞

RAGE, receptor for advanced glycation end products; F, forward; R, reverse; PCR, polymerase chain reaction.