Table I.
Name | Primer sequence (5′-3′) | Size of PCR product (bp) | Thermocycling protocol |
---|---|---|---|
RAGE | F: AGGTGAGTGGAGAAAGCCAG | 121 | 94°C for 3 min; |
R: ATGTGTCAGGTGTTTAATCA | 35 cycles of 94°C for 30 sec, 56°C for | ||
30 sec and 72°C for 30 sec; | |||
72°C for 7 min; | |||
4°C ∞ | |||
94°C for 3 min; | |||
GAPDH | F: AGGATGGTGTGGCTCCCTTG | 105 | 35 cycles of 94°C for 30 sec, 58°C for |
R: GCAGGGCTGAGACAGCTTCC | 25 sec and 72°C for 25 sec; | ||
72°C for 7 min; | |||
4°C ∞ |
RAGE, receptor for advanced glycation end products; F, forward; R, reverse; PCR, polymerase chain reaction.