RT-qPCR detection results of Bcl-2 mRNA. Results of analysis of variance show that the Bcl-2 mRNA expression levels in the 4 groups of rats at 6, 12 and 18 h after operation are different (P<0.05). In I°, II° and III burn groups, the Bcl-2 mRNA expression levels are decreased at 6, 12 and 18 h after surgery compared with those before modeling (P<0.05), and they are decreased at 12 and 18 h after operation compared with those at 6 h after operation (P<0.05) and also decreased at 18 h after operation compared with those at 12 h after operation (P<0.05). At the same time point, the Bcl-2 mRNA expression levels in I°, II° and III° burn groups are decreased compared with that in the SHAM group (P<0.05), and they are decreased at each time point in II° and III° burn groups compared with that in I° burn group (P<0.05) and also decreased at each time point in III° burn group compared with that in II° burn group (P<0.05).