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. 2019 Jan 15;17(3):2061–2070. doi: 10.3892/etm.2019.7176

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — E-cadherin increases in subcultured NSCs but decreases subsequent to induced differentiation. (A) Reverse transcription-quantitative polymerase chain reaction revealed that E-cadherin was significantly increased in P5 NSCs compared with P0 cells, and decreased in differentiated NSCs. (B) The change was further confirmed by western blotting. (C) Expression of β-catenin, the core component of E-cadherin/β-catenin complex, was increased in subcultured NSCs, as confirmed by flow cytometry. **P<0.01 as indicated or vs. P0-NSCs. NSC, neural stem cells; D-NSCs, differentiated NSCs; P0, primary cells; P5, passage 5.