Figure 6.

E-cadherin alleviated secondary damage by reducing the release of inflammatory cytokines. (A) The release of inflammatory cytokines (IL-1β, IL-6, IL-8, MCP-1, iNOS and TNF-α) was increased in the PBS group 3 days after transplantation. (B) INF-γ was able to induce the ectopic expression of IL-1β, iNOS and TNF-α released by activated macrophages, and NSCs alone were able to reduce the expression of these cytokines, while NSCs with E-cadherin exhibited the most marked inhibitory effect. (C) Cytokines were detected in NSCs alone in response to INF-γ treatment. No obvious difference was observed before and after INF-γ treatment. *P<0.05, **P<0.01 as indicated; ^##P<0.01 vs. NSCs. NSCs, neural stem cells; IL, interleukin; MCP-1, monocyte chemoattractant protein 1; iNOS, inducible nitric oxide synthase; TNF-α, tumor necrosis factor α; IFN-γ, type II interferon.