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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Methods Enzymol. 2018 Dec 8;615:237–284. doi: 10.1016/bs.mie.2018.09.010

Figure 9.

Figure 9.

Sampling statistics of measured O2axis of barnase binding DNA. The O2axis values of free barnase and barnase bound to DNA are analyzed to determine the robustness of Δ<O2axis> and <ΔO2axis> values. (A) Bootstrap analysis of <O2axis> of barnase (black circles) and barnase-DNA (grey squares) as a function of dataset size, showing a narrow distribution of possible <O2axis> of 0.669 ± 0.026 and 0.647 ± 0.028, respectively. (B) Jackknife analysis of <O2axis> identifies the extra data point not measured in the barnase-DNA dataset by removing it and resulting in one of the extreme <O2axis> values using n-1 datapoints (open circle). (C) Bootstrap analysis of <ΔO2axis> of barnase binding DNA, yielding the value −0.013 ± 0.008. The value is well-determined even when only 20 datapoints are used. The global difference Δ<O2axis> is shown as a black circle. (D) Jackknife analysis of site-specific <ΔO2axis> shows a narrow distribution of possible values.