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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Methods Enzymol. 2018 Dec 8;615:237–284. doi: 10.1016/bs.mie.2018.09.010

Table 1.

General guidelines for selecting the type of relaxation experiment suitable for a given protein system of interest. Based on our laboratory’s experiences and data collection at 25˚C

Experiment Molecular Weight Sample Concentration High Resolution Structure
Full Ansiotropy Analysis < 25 kDa > 0.5 mM Required
2H R1, R1ρ < 25 kDa > 0.5 mM Not Required
TROSY 2H > 25 kDa > 0.5 mM Not Required
13C R1, R1p Small or Large > 0.1 mM Required
1H-1H Cross Correlated > 25 kDa > 0.2 mM Not Required