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. Author manuscript; available in PMC: 2019 Feb 6.
Published in final edited form as: ACS Biomater Sci Eng. 2017 Sep 11;3(12):3128–3132. doi: 10.1021/acsbiomaterials.7b00561

Figure 4.

Figure 4.

Peptide nanofibers efficiently delivered CD8+ T cell epitopes to APCs and stimulated CD8+ T cell responses. (a) Representative flow cytometry data showing SIINFEKL/MHC-I positive dendritic cells 20 h after i.p. injections of SIINFEKL/Alum, SIINFEKL-Coil29, or SIINFEKL-Q11 (b) Quantification of SIINFEKL presentation in MHC-I by DCs. SIINFEKL-Coil29 immunization led to a significantly larger proportion of SIINFEKL-presenting DCs compared with SIINFEKL-Q11 and SIINFEKL/Alum (N=3 mice per group, p<0.05, analyzed by two-way ANOVA for multiple comparison.) (c) Splenocytes harvested from mice immunized with SIINFEKL/CFA, SIINFEKL-Coil29, or SIINFEKL-Q11 exhibited comparable IFNγ responses when restimulated with SIINFEKL peptide. (# statistically insignificant, N=12 mice per group, analyzed by two-way ANOVA for multiple comparison.)