Skip to main content
. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Transgenic Res. 2018 Oct 23;27(6):559–569. doi: 10.1007/s11248-018-0098-6

TABLE 1.

Percentage recombination efficiency in CrymCh;−1.9mylz2:CreERT2; ubi:Switch embryos treated with 0.5μM, 5μM and 10μM 4-OHT at 10-somites. EGFP and mCherry fluorescence in somites 11-13 (marked 1, 2, 3) was quantified in five embryos for each concentration of 4-OHT.

induction at 10-somites and somites 11-13 imaged at 72 hpf
4-OHT conc. (μM) Fish No. % recombination efficiency Average (% recombination efficiency) ± SD
0.5 1 44.15 40.63 ± 4.10
2 39
3 45.11
4 39.91
5 35
5 1 53.90 48.02 ± 7.47
2 49.80
3 39.34
4 41.12
5 55.91
10 1 79.85 78.70 ± 8.73
2 91.04
3 74
4 81.04
5 67.59