Fig. 6.

CD147 regulates the p38 MAPK/MMP9 signal pathway and is involved in CS-induced MUC5AC secretion in HBE cells. Cells were transfected with CD147 siRNA and pretreated with 10 μM SB203580 (p38 MAPK inhibitor) or 400 nM SB-3CT (MMP9 inhibitor) for 1 h; then, the cells were stimulated with 10% CS. After 24 h, MUC5AC protein (a) and mRNA (b) levels were detected. *p < 0.05, compared with the CS extract treatment group, **p < 0.05, compared with the CS + CD147 siRNA treatment group. Cell extracts were subjected to Western blotting analysis to detect MMP, p-p38 MAPK and total p38 MAPK (c, d and e). ^#p < 0.05, compared with the CS extract treatment group, ^##p < 0.05, compared with the CS + CD147 siRNA treatment group, ***p > 0.05, compared with the CS+ CD147 siRNA treatment group, ^###p > 0.05 compared with the CS extract treatment group