Fig. 4.

ZEB2 is a downstream target of TRIM14. a Western blot analysis of TRIM14, EMT related biomarks (Snail1, Twist1, ZEB1, ZEB2 and SLUG), and β-actin in LN229 and U251 cells transfected with shTRIM14–1, shTRIM14–2 or control shRNA. b Western blot analysis of TRIM14, N-cadherin, E-cadherin, Vimentin and β-actin in LN229 and U251 cells transduced with the indicated plasmid. c Representative images of transwell invasion assay using LN229 and U251 cells transfected with the indicated plasmid. Quantification of transwell invasion assay is shown. ***p < 0.001; n = 3 experiment. d Representative images of wound healing assay using LN229 and U251 cells with the indicated plasmid. Quantification of wound healing assay is shown. ***p < 0.001, n = 3 experiments. e Representative images of 3D spheroid BME cell invasion assays using LN229 and U251 cells with the indicated plasmid. Quantification of 3D spheroid BME cell invasion assays is shown. ***p < 0.001, n = 3 experiments