Skip to main content
. Author manuscript; available in PMC: 2019 Feb 6.
Published in final edited form as: Science. 2017 May 12;356(6338):eaal3118. doi: 10.1126/science.aal3118

Figure 2. Altered microvillar cumulative coverage in response to ligand detection.

Figure 2.

(A) Left: Image of a T cell in interaction with a peptide-loaded APC (unlabeled). The non-synaptic (non-IS) plane of interest is outlined in blue, the synapse (IS) is outlined in green. (See also Movie 2 for another example of an immunological synapse formed between a T cell and an APC with both cells labeled.) Right: Thresholded images of the non-IS (blue) and IS (green) contact face at a single timepoint and a cumulative image when coverage had reached 75%. (B) Left: Percent surface coverage throughout time for a non-IS and IS region of a single T cell. Right: The distribution of average surface coverage for non-IS regions and IS regions taken from multiple T cells. Error bar corresponds to SD, data are pooled from 4 separate experiments. (C) Percent cumulative coverage comparing the non-IS and IS region of a single T cell. Line indicates 75% surface coverage. (D) Comparison between the distributions of T75% for isolated T cells, IS regions with no antigen, non-IS regions, and IS regions of multiple individual T cells. Error bar corresponds to SD, data are pooled from 4 separate experiments. The significance test was unpaired t-test.