Figure 6.

OCR and fatty acid oxidation in H9c2 cells expressing PR-M. Cells expressing PR-M treated with 10⁻⁶ M ligand R5020 (PRM-R5020) for 48 h showed increased (A) basal OCR and (C) ATP-linked OCR, whereas (B) maximum OCR and (D) spare capacity approached significance (P = 0.06). (E) There was no difference in proton leak. (F) With the same treatment, cells expressing PR-M showed increased oxidation of tritiated palmitate, with a positive control of bezafibrate and a negative control of etomoxir. Comparison treatments included control plasmid–transfected cells treated with vehicle (Ctl-EtOH), control plasmid cells treated with R5020 (Ctl-R5020), and PRM-expressing plasmid treated with vehicle (PRM-EtOH). Results are expressed in scatter plots with bar as mean. Treatments with different lettered superscripts are statistically different. cpm, counts per min.