Figure 3.

The eggshells and saline extract of the eggshells inhibit biofilm formation and bacterial survival. (a) The viability of E. coli culture in the presence of 20 eggshells or 1 µg µl⁻¹ kanamycin compared to control. Bacterial culture without the addition of eggshells was used as negative control. (b) Biofilm production of K. oxytoca, P. aeruginosa and M. morganii inoculums with or without 50 eggshells (ES). SEM analysis of water spheres extract encased by organic material (c) or not (d). Saline extract of eggshells (2 µl) was tested for its bacterial growth inhibition capability against M. morganii (e) and S. aureus (f). (Online version in colour.)