Figure 5.

Oviposition fluid is similar in composition to eggshell water extract. (a) MALDI-MS analysis of the oviposition fluid. (b) Coomassie-blue-stained SDS-PAGE of oviposition fluid. Arrows show the similarity of the m/z masses of the MALDI-MS analysis to the SDS-PAGE protein profile. (c) SEM image of oviposition fluid dried on a silica chip. (d) The white crystals present in the oviposition fluid were identified by SEM composition analysis as KCl crystals. (e) SEM analysis of the silica chip surface indicates that the origin of the KCl crystals is the oviposition fluid. (Online version in colour.)