Fig. 1. MOG_35–55-primed, but not OVA_323–339-primed, Th cells accumulate in the meninges and CNS early in EAE.

Four x10⁶ MOG_35–55- or OVA_323–339-primed T cell blasts from Thy1.1⁺ donor mice were restimulated with peptide in vitro and transferred to congenic Thy1.2⁺ recipients. The frequency and numbers of Thy1.1⁺CD45⁺CD4⁺ cells in the meninges (a,c) and CNS (b,c) was determined 3 or 6 days post transfer. (a) Representative analysis of MOG_35–55 or OVA_323–339-primed CD4⁺Thy1.1⁺ T cells detected in the pooled meninges of Thy1.2⁺ recipients at day 6 post transfer. (b) Representative analysis of MOG_35–55 or OVA_323–339-primed CD4⁺Thy1.1⁺ T cells detected in the CNS (pooled brain and spinal cord) of Thy1.2⁺ recipients 6 days post transfer. Percentages of the CD45^+/hi parent gate are shown. (c) Numbers of CD45⁺CD4⁺Thy1.1⁺ MOG_35–55 or OVA_323–339-primed T cells in the meninges and CNS at indicated days post T cell transfer. For meninges samples, each point represents the analysis of a pool of tissues from 3 to 5 mice and is expressed as numbers/mouse. CNS data points represent the analysis of individual mice. *p < 0.05 and **p < 0.01 by Student’s t-Test. 4 independent experiments.