Fig. 3.

Robust GM-CSF production by Th cells is acquired post priming in wild type, but not Kit^W/Wv mice. (a) Representative analysis of cytokine production by MOG_35–55 eprimed wild type donor T cell blasts prior to adoptive transfer. Numbers denote frequency of cytokine-expressing CD3⁺CD4⁺ blast cells and are representative of 3 independent experiments. (b) Accumulation of CD45⁺CD3⁺CD4⁺ T cells in the meninges at day 12 post transfer of 4 × 10⁶ Thy1.1⁺ T blasts to wild type (WT) and Kit^W/Wv (W/W^v) recipients. Each data point represents analysis of meningeal tissue pooled from 3 mice and is expressed as average cell number per mouse. 3 independent experiments. **p < 0.01 by Student’s t-Test. (c) Representative analysis showing the frequency of Thy1.1⁺ T cells of the CD45⁺CD3⁺CD4⁺ population in the meninges of indicated recipients at day 12 post T cell transfer. (d) Accumulation of CD45^hiCD3⁺CD4⁺ T cells in the CNS of indicated recipients at day 12 post T cell transfer. Each data point represents analysis of pooled brain and spinal cord tissue from one mouse, 3 independent experiments. *p < 0.05 by Student’s t-Test. (e) Representative analysis showing the frequency of Thy1.1⁺ T cells of the CD45^hiCD3⁺CD4⁺ population in the CNS of indicated recipients. (f) Representative analysis of cytokine production by CD45⁺CD3⁺CD4⁺ Th cells in the meninges of pooled samples from 4 mice at day 12 post transfer of T cell blasts. Numbers denote frequency of cytokine expressing CD45⁺CD3⁺CD4⁺ cells in WT (black) and W/W^v (green) meninges. (g) Representative analysis of cytokine production by CD45^hiCD3⁺CD4⁺ T cells in the CNS of indicated recipients at day 12 post transfer of T blasts. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)