Figure 1.

Arctic mutant expression leads to rapid protein aggregation. A, cells expressing mCherry show a homogeneous distribution of soluble reporter protein. B, cells expressing mCherry-Aβ42(WT) generate intracellular aggregates but only in a small proportion of cells. C, cells expressing mCherry-Aβ42(E22G) show the development of aggregates in most cells. D, proportion of cells containing aggregates, assessed by wide-field microscopy, at different time points after tetracycline induction of gene expression. Three independent experiments were performed with around 200 cells assessed per population per experiment. D0, day 0; D1, day 1 (24 h after gene induction), etc. Error bars represent S.D.