Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 5;294(5):1554–1567. doi: 10.1074/jbc.RA118.006128

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Gunther et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 7. — Transient time-resolved FRET: time-resolved fluorescence waveform acquisition of structural kinetics. A, (TR)²FRET of the recovery stroke. The fluorescence of 160 nm MV FlAsH.QSY-CaM changes upon rapid stopped-flow mixing with varying concentrations of ATP (0.01 mm shown). Both the peak fluorescence intensity and the waveform shape change over the reaction time. The three-dimensional plot depicts the nanosecond-resolved waveforms (xy-plane) evolving with reaction time (z axis). Each waveform was fit to a two-Gaussian distance distribution. The three-dimensional plot depicts each distance distribution (xy-plane) evolving with reaction time (z axis). B, the summary of rate constants from fitting the recovery-stroke (TR)²FRET data to a double-exponential function. C, (TR)²FRET of the power stroke. 160 nm MV and 10 μm ATP were mixed with 5.0 mm ADP and varying actin concentrations. D, summary of rate constants from fitting the power-stroke (TR)²FRET data to a single-exponential function.