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All aSec proteins required for transport bind anionic lipids, but not the SRR protein-specific glycosyltransferases. Shown is lipid binding of aSec transport and glycosylation proteins with PC liposomes (a), PC/MGDG liposomes (b), PC/CL liposomes (c), and PC/PG liposomes (d). Representative Coomassie-stained SDS-polyacrylamide gels are shown.
