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. 2018 Dec 4;294(5):1502–1515. doi: 10.1074/jbc.RA118.005657

Figure 6.

Figure 6.

Lipid binding by the GspB signal peptide is required for SecA2 to bind GspBvD in vivo. a, cross-linking of Bpa-labeled GspBvD in E. coli (GspBvD-Bpa) with or without SecA2 expression and with or without UV treatment. b, cross-linking of GspBvD-Bpa mutants with SecA2 in E. coli with or without UV treatment. GspBvD and cross-linked products were detected by Western blot analysis using an anti-FLAG antibody as described under “Experimental procedures.” *, SecA2-GspBvD-Bpa cross-linked product; **, all GspBvD-Bpa variants except Δ8–68; ***, GspBvD-Bpa Δ8–68. Splice sites are indicated by thick black lines after lanes 4, 6, and 8.