Table 1.
Plasmids used in the present study
| Plasmids | Relevant characteristics | Reference or source |
|---|---|---|
| pVA891 | Streptococcal integration shuttle vector | Ref. 47 |
| pB736FLAG-R | gspB736FLAG and pVA891 rescued from PS919 | Ref. 16 |
| pB736FLAG-R Δ8–20 | pB736FLAG-R with deletion of codons 8–20 | This study |
| pB736FLAG-R Δ20–28 | pB736FLAG-R with deletion of codons 20–28 | This study |
| pB736FLAG-R Δ28–36 | pB736FLAG-R with deletion of codons 28–36 | This study |
| pB736FLAG-R Δ36–44 | pB736FLAG-R with deletion of codons 36–44 | This study |
| pB736FLAG-R Δ44–52 | pB736FLAG-R with deletion of codons 44–52 | This study |
| pB736FLAG-R Δ52–60 | pB736FLAG-R with deletion of codons 52–60 | This study |
| pB736FLAG-R Δ60–68 | pB736FLAG-R with deletion of codons 60–68 | This study |
| pB736FLAG-R Δ8–68 | pB736FLAG-R with deletion of codons 8–68 | Ref. 16 |
| pB736FLAG-R ΔRK | pB736FLAG-R with Ala substitutions of Lys and Arg codons between codons 1–68 | This study |
| pCDFduet-1 | E. coli T7 expression vector | Novagen |
| pCDF.GspBvD.His6 | Vector expressing GspBvD-His6 fusion protein | This study |
| pCDF.GspBvD Δ1–90.His6 | Vector expressing GspBvD-His6 fusion protein with deletion of codons 1–90 | This study |
| pET28c | E. coli T7 expression vector | Novagen |
| pET28c.SP-AST.His6 | Vector expressing GspB SP-AST-His6 fusion protein | This study |
| pET28a.SP-AST.His6 Δ8–20 | pET28c.SP-AST.His6 with deletion of codons 8–20 | This study |
| pET28a.SP-AST.His6 Δ20–28 | pET28c.SP-AST.His6 with deletion of codons 20–28 | This study |
| pET28a.SP-AST.His6 Δ28–36 | pET28c.SP-AST.His6 with deletion of codons 28–36 | This study |
| pET28a.SP-AST.His6 Δ36–44 | pET28c.SP-AST.His6 with deletion of codons 36–44 | This study |
| pET28a.SP-AST.His6 Δ44–52 | pET28c.SP-AST.His6 with deletion of codons 44–52 | This study |
| pET28a.SP-AST.His6 Δ52–60 | pET28c.SP-AST.His6 with deletion of codons 52–60 | This study |
| pET28a.SP-AST.His6 Δ60–68 | pET28c.SP-AST.His6 with deletion of codons 60–68 | This study |
| pET28a.SP-AST.His6 Δ8–68 | pET28c.SP-AST.His6 with deletion of codons 8–68 | This study |
| pET28a.SP-AST.His6 ΔRK | pET28c.SP-AST.His6 with Ala substitutions of Lys and Arg codons between codons 1–68 | This study |
| pMAL-c2x | E. coli T7 expression vector | New England Biolabs |
| pMAL.MBP.His6.SecA2 | Vector expressing MBP-His6-SecA2 fusion protein | This study |
| pET21b | E. coli T7 expression vector | Novagen |
| pET21b.Asp1.GST.His6 | Vector expressing Asp1-GST-His6 fusion protein | Ref. 26 |
| pMal.Asp2-S362A | Vector expressing MBP-Asp2 fusion protein | Ref. 22 |
| pQE-TriSystem His-Strep 2 | E. coli expression vector | Qiagen |
| pET28c.StrepII.Asp3.His8 | Vector expressing StrepII-Asp3-His8 fusion protein | This study |
| pBAD.HisA | E. coli expression vector | Invitrogen |
| pBAD.His6.GtfAB | Vector expressing His6-GtfA and GtfB complex | Ref. 19 |
| pET28a | E. coli T7 expression vector | Novagen |
| pET28a.His6.Nss | Vector expressing His6-Nss fusion protein | This study |
| pET28a.His6.Gly | Vector expressing His6-Gly fusion protein | This study |
| pET28c.SecA2 | Vector expressing His6-SecA2 fusion protein | Ref. 39 |
| pEVOL.pBpF | Vector expressing amber codon tRNA synthetase/tRNA pair | Ref. 48 |
| pCDF.GspBvD-Q94X | Vector expressing GspBvD with TAG amber codon at codon 94 | Ref. 18 |
| pCDF.GspBvD-Q94X Δ8–20 | pCDF.GspBvD-Q94X with deletion of codons 8–20 | This study |
| pCDF.GspBvD-Q94X Δ20–28 | pCDF.GspBvD-Q94X with deletion of codons 20–28 | This study |
| pCDF.GspBvD-Q94X Δ28–36 | pCDF.GspBvD-Q94X with deletion of codons 28–36 | This study |
| pCDF.GspBvD-Q94X Δ36–44 | pCDF.GspBvD-Q94X with deletion of codons 36–44 | This study |
| pCDF.GspBvD-Q94X Δ60–68 | pCDF.GspBvD-Q94X with deletion of codons 60–68 | This study |
| pCDF.GspBvD-Q94X Δ8–68 | pCDF.GspBvD-Q94X with deletion of codons 8–68 | This study |
| pCDF.GspBvD-Q94X ΔRK | pCDF.GspBvD-Q94X with Ala substitutions of Lys and Arg codons between codons 1 and 68 | This study |