Table 2.
Strains used in the present study
| Strains | Relevant characteristicsa | Reference or source |
|---|---|---|
| S. gordonii | ||
| M99 | Parental stain | |
| PS846 | M99 ΔgspB::pEVP3, Cmr | Ref. 16 |
| PS1740 | M99 ΔgspB | Ref. 12 |
| PS961 | PS846 ΔgspB::pB736FLAG-R, Cmr Ermr | Ref. 16 |
| PS3701 | PS1740 ΔgspB::pB736FLAG-R Δ8–20 Ermr | This study |
| PS3051 | PS1740 ΔgspB::pB736FLAG-R Δ20–28, Ermr | This study |
| PS3702 | PS846 ΔgspB::pB736FLAG-R Δ28–36, Cmr Ermr | This study |
| PS3052 | PS1740 ΔgspB::pB736FLAG-R Δ36–44, Ermr | This study |
| PS3703 | PS846 ΔgspB::pB736FLAG-R Δ44–52, Cmr Ermr | This study |
| PS3704 | PS846 ΔgspB::pB736FLAG-R Δ52–60, Cmr Ermr | This study |
| PS3705 | PS846 ΔgspB::pB736FLAG-R Δ60–68, Cmr Ermr | This study |
| PS1027 | PS846 ΔgspB::pB736FLAG-R Δ8–68, Cmr Ermr | Ref. 16 |
| PS3706 | PS1740 ΔgspB::pB736FLAG-R ΔRK, Ermr | This study |
| E. coli | ||
| TOP10 | Host for cloning | Invitrogen |
| XL10-Gold | Host for cloning | Agilent |
| BL21(DE3) | F− ompT gal dcm lon hsdSB (rB−mB−)λ (DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12 (λS) | Novagen |
| C43(DE3) | F− ompT gal dcm hsdSB (rB− mB−) (DE3) | Lucigen |
a Cmr, chloramphenicol resistance; Ermr, erythromycin resistance.