Fig. (6).

IVIG regulates plasma levels of mRNAs encoding calcium-signaling molecules. Three months of IVIG altered plasma levels of select mRNAs regulating calcium-dependent intracellular signaling, synaptic plasticity, and cell survival. A) Heat map (red to green = increasing mRNA levels) shows that, compared to placebo, IVIG treatment resulted in 35–45% higher plasma levels of transcripts encoding calcium/calmodulin-mediated protein kinase 2A (CAMK2A), protein kinase C, alpha (PRKCA), protein kinase C, iota (PRKCI), and inositol triphosphate kinases A and B (ITPKA and ITPKB). By contrast, mRNA levels of voltage-gated calcium channel, N-type (CACNA1B) were decreased 35% with IVIG compared to placebo. Levels of these transcripts were not significantly different between the baseline and IVIG groups. B) Six months of IVIG resulted in ~50% higher levels of CAMK2A, PRKCA, PRKCI, ITPKA, and ITPKB. CREB transcripts levels were also increased 30% compared to placebo. Plasma CACNA1B levels were decreased 40% with IVIG compared to placebo. Levels of these transcripts were not significantly different between the baseline and IVIG groups. n = 12/group; *, p< 0.01, **, p< 0.001 via one-way ANOVA with Bonferroni’s post hoc test for multiple comparisons. Other abbreviations: (ERK1 (extracellular signal related kinase 1), AKT1 (v-akt viral oncogene homolog 1), CREB (cAMP-response element binding protein), PRKCE (protein kinase C, epsilon), ITPKC (inositol triphosphate kinase C), CACNA1A (voltage-gated calcium channel, P/Q-type), CACNA1C (voltage-gated calcium channel, L-type).