Fig 2. Relationship between the number of Cronartium ribicola (A), Melampsora medusae f. sp. deltoidae (B) and Melampsora larici-populina (C) spores from which DNA was isolated and C_t values, as determined by real-time PCR.

Each dot represents a biological independent replicate (corresponding to a known amount of spores), which was obtained by averaging the C_t values of three technical real-time PCR replicates.