. 2019 Feb 6;14(2):e0210952. doi: 10.1371/journal.pone.0210952

Table 1. Genus- and species-specific assays for hierarchical detection of Cronartium spp. and Cronartium ribicola.

Assay	Targeted taxon	Primer or TaqMan probe^a	Sequence (5’ → 3’)	Tm (NN)^b	Amplicon length	GenBank accession no.
CRO30	Cronartium spp.	Cronartium30F	`ATCGTTCTCATCGTCAGCG`	57.1 °C	104 bp	MH171752-MH171772
		Cronartium30R	`TTAAAGCTTTGAAGGCGACG`	56.0 °C
		Cronartium30T (47–70)	`TCTTCACTGCCGTCTACTTTCTGC`	64.2 °C
CRO46	Cronartium spp.	Cronartium46F	`CCGGTTATGGCGATCTCAC`	60.4 °C	117 bp	MH171773-MH171780
		Cronartium46R	`AAATCGGGCACTCTGTTCTG`	58.9 °C
		Cronartium46T (72–98)	`ATCGCTTTCGATGGACGTGATCATGGC`	67.4 °C
CRIB65	Cronartium ribicola	Crib65Fm	`CTTTTGCTACTTGCGCATTTGA`	57.0 °C	102 bp	MH171727-MH171734
		Crib65Rm	`CTCTGGTGAGGAGCTATCG`	60.1 °C
		Crib65T (61–83)	`CTACAGGGTTCACCTGCTTCGCC`	68.7 °C
CRIB146	Cronartium ribicola	Crib146F	`TCAGGTTTCGGATTTTGAGG`	55.8 °C	115 bp	MH171735-MH171742
		Crib146R	`TGCTTCTGAGGCTTTTTGGT`	58.5 °C
		Crib146T (37–63)	`CATACCTGATACGAGTGGACTGAATGA`	63.6 °C
CRIB190	Cronartium ribicola	Crib190Fm	`CTCCAGCTACAGTGGGTA`	59.9 °C	121 bp	MH171743-MH171751
		Crib190Rm	`CCTTGTCTGTTGGTGAGGT`	59.2 °C
		Crib190T (50–77)	`ACATGGGAACGACAAGGACAATTTGGAC`	66.0 °C

^a In parentheses: probe position within amplicon.

^b Values estimated by Oligo Analyzer V1.5.