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. 2019 Jan 25;15(1):e1007489. doi: 10.1371/journal.ppat.1007489

Fig 4. LANA oligomerization interface is required for LANA body formation and colocalization with DAXX and EZH2.

Fig 4

(A) Schematic of KSHV Bac-RFP-LANA WT and Bac-RFP-LANA MT (F1037A/F1041A). (B) Quantification of RFP-LANA nuclear body formation in iSLK stable clones for WT and MT LANA. P-value was determined by two-tailed student t-test. (C) Indirect immunofluorescence analysis of the association of RFP-LANA (red) with DAXX (green) in RFP-LANA WT1 and WT2, or MT1 or MT2 stable iSLK cell lines. Cells were counter-stained with Dapi (blue) as shown in merge. Scale bar = 10 μm. (D) The same as in panel C, except imaging with EZH2 (green). Scale bar = 10 μm.