Table 1. In the absence of Ikaros, activated CD4 T cells display up-regulation of pathways linked to inflammation and autoimmunity.

Results of GSEA analyses of microarray data for Ik^flox x dLck-Cre [Cre+] and Ik^flox (no Cre) [Cre] CD4 T cells activated with αCD3/soluble αCD28 for 24 hours. In total, 1876 gene sets were significantly enriched in Cre+ relative to Cre- CD4 T cells (FDR q<0.25). Examples of enhanced biological processes, functions and pathways are shown. ES: Enrichment score; NES: Normalized enrichment score.

Gene Set Name	Gene Set Size	ES	NES	Nominal p value	FDR q value
Hallmark TNFA Signaling via NFKB	192	0.67	2.72	<0.001	0.0000
Hallmark IL2 STAT5 signaling	196	0.59	2.41	<0.001	0.0000
Hallmark Interferon Gamma Response	182	0.59	2.41	<0.001	0.0000
Hallmark Inflammatory Response	189	0.58	2.37	<0.001	0.0000
GO Response to Interferon Gamma	104	0.62	2.36	<0.001	0.0000
Biocarta Cytokine Pathway	18	0.89	2.34	<0.001	0.0000
KEGG Type I Diabetes Mellitus	31	0.76	2.29	<0.001	0.0001
Biocarta Inflammatory Pathway	25	0.77	2.20	<0.001	0.0005
KEGG Graft versus Host Disease	23	0.77	2.21	<0.001	0.0006
KEGG Allograft Rejection	25	0.77	2.18	<0.001	0.0010
GO Response to Interferon Beta	20	0.79	2.11	<0.001	0.0022
Hallmark Interferon Alpha Response	87	0.58	2.08	<0.001	0.0030
GO Negative Regulation of Viral Genome Replication	35	0.68	2.06	<0.001	0.0037
KEGG Autoimmune Thyroid Disease	27	0.69	2.02	<0.001	0.0057