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. Author manuscript; available in PMC: 2019 Feb 6.
Published in final edited form as: Cell Rep. 2019 Jan 22;26(4):933–944.e4. doi: 10.1016/j.celrep.2019.01.002

Figure 2. Ex-miRNA Are Stable and Protected from RNases in BALF.

Figure 2.

(A) qPCR of synthetic miRNAs (“calibrators” Cal1 and Cal2) spiked into Trizol or BALF with or without an RNase inhibitor (RNAsin) (n = 3 from 1–2 independent experiments, 1-way ANOVA with Bonferroni’s multiple comparison test).

(B) qPCR of analysis of miRNAs from BALF and RNA pellets treated with RNaseA (n = 3 from 3 independent experiments, 2-way ANOVA with Bonferroni’s multiple comparison test).

Error bars are mean + SD; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.