KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-Alix [EPR15314] N-terminal | Abcam | Cat# ab186429; RRID:AB_2754981 |
| Rabbit polyclonal anti-Tsg101 | Abcam | Cat# ab30871; RRID:AB_2208084 |
| Rabbit monoclonal anti-CD63 [EPR21151] | Abcam | Cat# ab217345; RRID:AB_2754982 |
| Rabbit polyclonal anti-Syntenin | Abcam | Cat# ab19903; RRID:AB_445200 |
| Rabbit polyclonal anti-Arf6 | Abcam | Cat# ab77581; RRID:AB_2058475 |
| Rat monoclonal anti-CD9 (KMC8.8) | Santa Cruz | Cat# sc-18869; RRID:AB_2076043 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP | Invitrogen | Cat# A16110; RRID:AB_2534782 |
| Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP | Invitrogen | Cat# A18745; RRID:AB_2535522 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| BD Microtainer SST | Becton Dickinson | Cat# 365967 |
| Imject Alum | Thermo Scientific | Cat# 77161 |
| Ovalbumin | Sigma-Aldrich | Cat# A5503; CAS Number 9006-59-1 |
| Phusion High-Fidelity DNA Polymerase | New England Biolabs | Cat# M0530 |
| RNase A | Thermo Scientific | Cat# EN0531 |
| RNasin | Promega | Cat# N2511 |
| Roche FastStart Universal SYBR Green Master mix (Rox) | Sigma-Aldrich | Cat# 04913850001 |
| TRIzol | Invitrogen | Cat# 15596018 |
| TRIzol LS | Invitrogen | Cat# 10296028 |
| Critical Commercial Assays | ||
| DNA Adenylation Kit | New England BioLabs | Cat# E2610 |
| miR-X First Strand Synthesis Kit | Clonetech | Cat# 638315 |
| Pierce BCA Protein Assay Kit | Thermo Scientific | Cat# 23227 |
| RNA 6000 Pico Kit | Agilent | Cat# 5067-1513 |
| Deposited Data | ||
| miRNA sequencing data | GEO | GSE121818 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Actin-cre (B6N.FVB-Tmem163Tg(ACTB-cre)2Mrt/CjDswJ) | The Jackson Laboratory | RRID:IMSR_JAX:019099 |
| Mouse: mTmG (B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J) | The Jackson Laboratory | RRID:IMSR_JAX:007676 |
| Mouse: Nkx2.1-cre (C57BL/6J-Tg(Nkx2-1-cre)2Sand/J) | The Jackson Laboratory | RRID:IMSR_JAX:008661 |
| Mouse: Vav-cre (B6.Cg-Commd10Tg(Vav1-icre)A2Kio/J) | The Jackson Laboratory | RRID:IMSR_JAX:008610 |
| Mouse: Wild type (C57BL/6J) | NCI Mouse Repository | Cat# 01B96 |
| Oligonucleotides | ||
| See Table S5 | N/A | |
| Software and Algorithms | ||
| cutadapt | Martin, 2011 | https://cutadapt.readthedocs.io/en/stable/installation.html |
| DESeq2 | Love et al., 2014 | https://www.bioconductor.org/packages/release/bioc/html/DESeq2.html |
| DREAM | Bailey, 2011 | http://meme-suite.org/doc/dreme.html |
| exceRpt small RNA-seq Pipeline v4 | Bioinformatics Research Laboratory, Baylor College of Medicine | http://www.genboree.org/site/ |
| FastX Toolkit | Cold Spring Harbor Laboratory | http://hannonlab.cshl.edu/fastx_toolkit/index.html |
| FlowJo v9 and v10 | TreeStar | FlowJo, RRID:SCR_008520; https://www.flowjo.com/solutions/flowjo |
| ImageJ | Schneider et al., 2012 | ImageJ, RRID:SCR_003070; https://imagej.net/Welcome |
| Improbizer | University of California, Santa Cruz | https://users.soe.ucsc.edu/~kent/improbizer/improbizer.html |
| Prism 7 | GraphPad Software | GraphPad Prism, RRID:SCR_002798; https://www.graphpad.com/ |
| R Studio | RStudio Team, 2017 | https://www.rstudio.com |