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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Mol Biol. 2018 Dec 21;431(3):576–592. doi: 10.1016/j.jmb.2018.12.010

Figure 3. Screening of substrate specificity of maize and moss ALDH12.

Figure 3.

(a) Measurements were performed with 1 mM substrates in 100 mM pyrophosphate buffer, pH 7.5 containing 3.0 mM NAD+. Specific activity values with GSAL and NAD+ of 32 nkat mg−1 for ZmALDH12 and 1.6 nkat mg−1 for PpALDH12 were arbitrarily taken as 100% for each isoform. Error bars stand for S.D. from four measurements. (b) Influence of pH on the catalytic activity of ZmALDH12 and PpALDH12. Measurements were performed with 1 mM GSAL and 3.0 mM NAD+ in Britton-Robinson buffers in the pH range of 6 – 10.