Skip to main content
. 2019 Jan 7;54(3):1001–1009. doi: 10.3892/ijo.2019.4678

Figure 2.

Figure 2

MAF1 is involved in the migratory ability of CRC cells. (A) Western blotting of MAF1 in CRC cell lines. (B) MAF1 expression levels in HCT 116 and RKO cells transfected with negative control siRNA or siRNAs against MAF1. mRNA expression levels were detected by reverse transcription quantitative polymerase chain reaction and were normalized to GAPDH. *P<0.001 (upper panel). Protein expression levels were detected by western blotting (lower panel). (C) Representative images of the scratch wound healing assay post transfection of HCT 116 and RKO cells with negative control siRNA or siRNAs against MAF1 (upper panel; magnification, ×100). Distance between wound edges at the indicated time points (normalized to distance at 0 h); the average of 10 dif ferent areas was obtained (lower panel). *P<0.05, **P<0.001 vs. negative control. CRC, colorectal cancer; MAF1, MAF1 homolog, negative regulator of RNA polymerase III; siRNA, small interfering RNA.