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(A) MCF-7 parent cells and MCF7 siRNA Hsp70 cells were cultured in 60 mm plates. RIPA cell extracts were selected and samples of 5, 10, 15, 20, 25 µl were analyzed by SDS-PAGE and then subjected to western blot analysis using the corresponding specific antibodies against the arrow-labeled proteins and the chemiluminescence method. (B) The used EMT markers.
