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HeLa, HeLa-siRNA-Hsp70, MCF7, MCF7-siRNA-Hsp70, A549, and A549-siRNA-Hsp70 cells were cultured in 60 mm plates. RIPA cell extracts were prepared, analyzed by SDS-PAGE and then subjected to western blot analysis, using specific antibodies for (A) E-cadherin, Hsp70, Vimentin and α-tubulin, or (B) against occludin, Slug and α-tubulin, and the enhanced chemiluminescence method.
